Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme can be used in a biosensor for malic acid detection in fruit juices, based on malate dehydrogenase (MDH), and glutamate oxaloacetate transaminase (GOT) in a powder vitreous carbon, carbon nanotubes and mineral oil, composite matrix | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a bienzymatic biosensor for malic acid based on malate dehydrogenase and transaminase immobilized onto a glassy carbon powder/carbon nanotubes/NAD+ composite electrode. In order to shift the conversion of malate to oxaloacetate, a reaction to convert oxaloacetate to L-aspartate is coupled to the main reaction, by reacting it with glutamic acid in the presence of glutamate oxaloacetate transaminase, GOT. The main reaction shifts to products, thus promoting the conversion of malate, thereby generating NADH, pH 10.0. Method overview | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Sus scrofa | - |
heart | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malate dehydrogenase | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa |